Till date theoretical calculation of the half maximal inhibitory concentration ( IC 50 ) of a compound is based on different Quantitative Structure Activity Relationship (QSAR) models which are empirical methods. © 2014 Society for Laboratory Automation and Screening.ĭevelopment of Quantum Chemical Method to Calculate Half Maximal Inhibitory Concentration ( IC 50 ). In each run, the system can handle up to 32 compounds and up to 10 concentration levels per compound, and it greatly improves IC 50/EC 50 assay experimental productivity and data processing efficiency. The automation-assisted IC 50/EC 50 assay platform can process the whole class of assays of varying assay conditions. In addition, the platform also automates the data extraction, nonlinear regression curve fitting, computation of IC 50/EC 50 values, graphing, and reporting. A general-purpose, automation-assisted IC 50/EC 50 assay platform was developed to automate the calculations of spiking solutions and the matrix solutions preparation scheme, the actual spiking and matrix solutions preparations, as well as the flexible sample extraction procedures after incubation. Existing bioanalytical sample preparation automation systems cannot meet IC 50/EC 50 assay throughput demand. Mass spectrometry (MS)-centric bioanalytical and biomarker labs are now conducting IC 50/EC 50 assays, which, if done manually, are tedious and error-prone. IC 50 and EC 50 values are commonly used to evaluate drug potency. Li, Ming Chou, Judy King, Kristopher W Jing, Jing Wei, Dong Yang, Liyu ICECAP: an integrated, general-purpose, automation-assisted IC 50/EC 50 assay platform.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |